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Human Suggested Panels Lists (Configurable)
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Species Specification Targets Human Human Adipokine Panel 10-plex Ⓢ Adiponectin/Acrp30, CCL2/JE/MCP-1, HGF, IL-1 beta/IL-1F2, IL-6, IL-8/CXCL8, Leptin/OB, beta-NGF, Serpin E1/PAI-1, TNF-alpha 상세보기 Human Human Angiogenesis Panel 15-plex Ⓢ Angiopoietin-2, BMP-9, EGF, Endoglin/CD105, FGF acidic/FGF1, FGF basic/FGF2/bFGF, G-CSF, HB-EGF, HGF, IL-8/CXCL8, Leptin/OB, PlGF, VEGF-A, VEGF-C, Endothelin-1 상세보기 Human Human Cancer Biomarker Panel 1 10-plex Ⓢ CA125/MUC16, CEACAM-5/CD66e, Fas/TNFRSF6/CD95, Fas Ligand/TNFSF6, FGF basic/FGF2/bFGF, HE4/WFDC2, HGF, IL-6, IL-8/CXCL8, Leptin/OB 상세보기 Human Human Cancer Biomarker Panel 2 10-plex Ⓢ alpha-Fetoprotein/AFP, CA15-3/MUC-1, MIF, Osteopontin/OPN, Prolactin, SCF/c-kit Ligand, TGF-alpha, TNF-alpha, TRAIL/TNFSF10, VEGF-A 상세보기 Human Human CD8 T Cell Panel 16-plex Ⓢ 4-1BB/TNFRSF9/CD137, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, Fas/TNFRSF6/CD95, Fas Ligand/TNFSF6, GM-CSF, Granzyme A, Granzyme B, IFN-gamma, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, TNF-alpha 상세보기 Human Human Cytokine/Chemokine Panel 37-plex Ⓢ CCL2/JE/MCP-1, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL7/MCP-3/MARC, CCL11/Eotaxin, CCL22/MDC, CD40 Ligand/TNFSF5, CX3CL1/Fractalkine, CXCL1/GRO alpha/KC/CINC-1, CXCL2/GRO beta/MIP-2/CINC-3, CXCL10/IP-10/CRG-2, EGF, FGF basic/FGF2/bFGF, Flt-3 Ligand/FLT3L, G-CSF, GM-CSF, IFN-gamma, IL-1 alpha/IL-1F1, IL-1 beta/IL-1F2, IL-1ra/IL-1F3, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IL-10, IL-12 p70, IL-15, IL-17/IL-17A, Lymphotoxin-alpha/TNF-beta, PDGF-AA, PDGF-BB, TGF-alpha, TNF-alpha, VEGF-A 상세보기 Human Human Cytokine/Chemokine 2 Panel 20-plex Ⓢ CCL1/I-309/TCA-3, CCL8/MCP-2, CCL13/MCP-4, CCL17/TARC, CCL24/Eotaxin-2/MPIF-2, CCL26/Eotaxin-3, CCL27/CTACK, CXCL5/ENA-78, CXCL13/BLC/BCA-1, IL-16, IL-21, IL-23, IL-28A/IFN-lambda 2, IL-33, LIF, SCF/c-kit Ligand, Thrombopoietin/Tpo, TRAIL/TNFSF10, TSLP, CCL21/6Ckine 상세보기 Human Human T Cell Panel 21-plex Ⓢ CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL20/MIP-3 alpha, CX3CL1/Fractalkine, CXCL11/I-TAC, GM-CSF, IFN-gamma, IL-1 beta/IL-1F2, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IL-10, IL-12 p70, IL-13, IL-17/IL-17A, IL-21, IL-23, TNF-alpha 상세보기 Human Human Th1/Th2 Panel 9-plex Ⓢ GM-CSF, IFN-gamma, IL-2, IL-4, IL-5, IL-10, IL-12 p70, IL-13, TNF-alpha 상세보기 Human Human Th17 Panel 21-plex Ⓢ CCL20/MIP-3 alpha, GM-CSF, IFN-gamma, IL-1 beta/IL-1F2, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p70, IL-13, IL-15, IL-17/IL-17A, IL-17E/IL-25, IL-21, IL-27, IL-28A/IFN-lambda 2, IL-31, IL-33, Lymphotoxin-alpha/TNF-beta, TNF-alpha 상세보기 Human Immuno-Oncology Checkpoint Panel 32-plex Ⓢ CCL2/JE/MCP-1, CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CD27/TNFRSF7, CD40/TNFRSF5, CD40 Ligand/TNFSF5, CXCL2/GRO beta/MIP-2/CINC-3, CXCL5/ENA-78, CXCL6/GCP-2, Galectin-1, Galectin-3, Galectin-9, GITR/TNFRSF18, IFN-gamma, IL-1 alpha/IL-1F1, IL-1 beta/IL-1F2, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8/CXCL8, IL-10, IL-12 p70, IL-15, IL-17/IL-17A, PD-L1/B7-H1, TNF-alpha, CCL7/MCP-3/MARC, IL-13, MICA 상세보기 Human Human Kidney Injury Panel 3-plex Ⓢ beta 2-Microglobulin, RBP4/Retinol-Binding Protein 4, Uromodulin 상세보기 Human Human MMP Panel 1 3-plex Ⓢ MMP-3, MMP-12, MMP-13 상세보기 Human Human MMP Panel 2 4-plex Ⓢ MMP-1, MMP-7, MMP-9, MMP-10 상세보기 Human Human Sepsis Panel 1 7-plex Ⓢ C-Reactive Protein/CRP, Fas/TNFRSF6/CD95, Fas Ligand/TNFSF6, ICAM-1/CD54, MIF, Serpin E1/PAI-1, VCAM-1/CD106 상세보기 Human Human Sepsis Panel 2 7-plex Ⓢ CCL3/MIP-1 alpha, CCL4/MIP-1 beta, Granzyme B, IL-1 alpha/IL-1F1, IL-8/CXCL8, MMP-8, Procalcitonin 상세보기 Human Human Soluble Cytokine Receptor Panel 11-plex Ⓢ CD30/TNFRSF8, CD25/IL-2R alpha, gp130, IL-1 RII, IL-4R alpha, IL-6R alpha, RAGE/AGER, TNF RI/TNFRSF1A, VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 상세보기 Human Human Cell Pyroptosis Panel 6-plex Ⓢ CASP1, CASP4, CASP5, NLRP3, GSDMD, NLRC4 상세보기 Human Human Mucosal Immunity Panel 3-plex Ⓢ MUC5AC, MUC5B, sIgA 상세보기 Human Human Osteogenic Factor Panel 8-plex Ⓢ SOST, OPG, BMP2, Osteocalcin, RUNX2, KlothoKL, MGP, S100A11 상세보기 Human Human Claudin Panel 8-plex Ⓢ CLDN1, CLDN2, CLDN3, CLDN4, CLDN5, CLDN6, CLDN8, CLDN18 상세보기 Human Human Amyloid Beta and Tau Panel 4-plex Ⓢ Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181) 상세보기 Human Human Neuroscience Panel 1 6-plex Ⓢ Glial Fibrillary Acidic Protei(GFAP), UCHL1/PARK5, DJ1/PARK7, Neuron-Specific Enolase (NSE), α-Synuclein, Transglutaminase 2 상세보기 Human Human Neuroscience Panel 2 6-plex Ⓢ Angiogenin, ApoE4, FABP3, Ferritin, Neurogranin, TREM2 상세보기 Human Human Neurodegenerative Disease Panel1 6-plex Ⓢ α2-Macroglobulin, Apo Al, Apo CIII, Apo E, Complement C3, Complement Factor H 상세보기 Human Human Neurodegenerative Disease Panel2 10-plex Ⓢ α1-Antitrypsin, Complement C4, CRP, MIP-4, PEDF, SAP 상세보기 Human Human Neurodegenerative Disease Panel3 10-plex Ⓢ BDNF, Cathepsin D, MPO, NCAM, PAI-1 (total), PDGF-AA, PDGF-AB/BB, RANTES, sICAM-1, sVCAM-1 상세보기 Human Human Neurodegenerative Disease Panel4 5-plex Ⓢ Amyloid β40, Amyloid β42, GDNF, sRAGE, S100B 상세보기 Human Human Neurological Disorders Panel3 7-plex Ⓢ AGT, Contactin-1, Fetuin A, Kallikrein-6, OPN, sSOD1, sSOD2 상세보기 Human Human Neuropeptide Panel 5-plex Ⓢ α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P 상세보기 Human Human Circadian/Stress Panel 2-plex Ⓢ Cortisol, Melatonin 상세보기 Human Human Exosome Characterization Panel 9-plex Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101 상세보기 Human Adipokine Panel 10-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) Adiponectin/Acrp30 44 B 823 - 200000 1:200 1:200 1:2 148 CCL2/JE/MCP-1 25 A 30.9 - 7500 1:2 1:2 1:2 9.9 HGF 66 C 16.5 - 4000 1:2 1:2 1:2 1.0 IL-1 beta/IL-1F2 57 A 17.7 - 4300 1:2 1:2 1:2 0.8 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 Leptin/OB 51 B 494 - 120000 1:2 1:2 1:2 10.2 beta-NGF 28 S 4.12 - 1000 1:2 1:2 1:2 0.259 Serpin E1/PAI-1 43 D 18.1 - 4400 1:200 1:200 1:2 0.7 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 Human Angiogenesis Panel 15-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) Angiopoietin-2 26 D 94.7 - 23000 1:2 1:2 1:2 17.1 BMP-9 73 C 2.06 - 500 1:2 1:2 1:2 0.6 EGF 25 C 8.23 - 2000 1:2 1:2 1:2 8.9 Endoglin/CD105 22 H 362 - 88000 1:2 1:2 1:2 68.0 FGF acidic/FGF1 67 J 16.5 - 4000 1:2 1:2 1:2 4.88 FGF basic/FGF2/bFGF 47 B 1.77 - 450 1:2 1:2 1:2 6.5 G-CSF 54 B 24.7 - 6000 1:2 1:2 1:2 4.1 HB-EGF 15 C 7.41 - 1800 1:2 1:2 1:2 0.4 HGF 66 C 16.5 - 4000 1:2 1:2 1:2 1.0 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 Leptin/OB 51 B 494 - 120000 1:2 1:2 1:2 10.2 PlGF 72 J 2.88 - 700 1:2 1:2 1:2 1.9 VEGF-A 61 A 11.5 - 2800 1:2 1:2 1:2 0.99 VEGF-C 38 F 57.6 - 14000 1:2 1:2 1:2 5.5 Endothelin-1 76 H 8.23 - 2000 1:2 1:2 1:2 1.31 * Endothelin-1 cannot be multiplexed with FGF basic/FGF2/bFGF, G-CSF, HGF, IL-8/CXCL8, EGF, Endoglin/CD105, Leptin/OB, VEGF-C, FGF acidic/FGF1, PlGF, Angiopoietin-2, or BMP-9 Human Cancer Biomarker Panel 1 10-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CA125/MUC16 35 F 24.7 - 6000 1:2 1:2 1:2 2.7 CEACAM-5/CD66e 30 N 61.7 - 15000 1:2 1:2 1:2 12.96 Fas/TNFRSF6/CD95 73 E 144 - 35000 1:2 1:2 1:2 3.2 FGF basic/FGF2/bFGF 47 B 1.77 - 450 1:2 1:2 1:2 6.5 HE4/WFDC2 62 O 172 - 42000 1:2 1:2 1:2 10.8 HGF 66 C 16.5 - 4000 1:2 1:2 1:2 1.0 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 Leptin/OB 51 B 494 - 120000 1:2 1:2 1:2 10.2 Fas Ligand/TNFSF6 39 D 8.23 - 2000 1:2 1:2 1:2 1.2 * Fas/TNFRSF6/CD95 cannot be multiplexed with Fas Ligand/TNFSF6 Human Cancer Biomarker Panel 2 10-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) alpha-Fetoprotein/AFP 66 H 823 - 200000 1:2 1:2 1:2 205 CA15-3/MUC-1 75 Z 0.144 - 35 1:50 1:50 1:2 0.051 MIF 21 F 556 - 135000 1:2 1:2 1:2 38.8 Osteopontin/OPN 54 J 1440 - 350000 1:2 1:2 1:2 413 Prolactin 36 K 1110 - 270000 1:2 1:2 1:2 224 SCF/c-kit Ligand 27 F 16.5 - 4000 1:2 1:2 1:2 1.4 TGF-alpha 63 L 6.17 - 1500 1:2 1:2 1:2 0.820 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 TRAIL/TNFSF10 37 K 28.8 - 7000 1:2 1:2 1:2 9.65 VEGF-A 26 A 11.5 - 2800 1:2 1:2 1:2 0.99 * Concentration for CA15-3 are in Units/mL. Human CD8 T Cell Panel 16-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) 4-1BB/TNFRSF9/CD137 13 P 28.8 - 7000 1:2 1:2 1:2 2.75 CCL3/MIP-1 alpha 35 A 90.5 - 22000 1:2 1:2 1:2 16.2 CCL4/MIP-1 beta 37 A 123 - 30000 1:2 1:2 1:2 5.8 Fas/TNFRSF6/CD95 73 E 144 - 35000 1:2 1:2 1:2 3.2 GM-CSF 46 B 12.3 - 3000 1:2 1:2 1:2 4.1 Granzyme A 47 N 24.7 - 6000 1:2 1:2 1:2 2.18 Granzyme B 57 L 19.3 - 4700 1:2 1:2 1:2 1.42 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-6 72 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-13 64 G 453 - 110000 1:2 1:2 1:2 32.4 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 Fas Ligand/TNFSF6 39 D 8.23 - 2000 1:2 1:2 1:2 1.2 * Fas/TNFRSF6/CD95 cannot be multiplexed with Fas Ligand/TNFSF6 Human Cytokine/Chemokine Panel 37-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CCL2/JE/MCP-1 62 A 30.9 - 7500 1:2 1:2 1:2 9.9 CCL3/MIP-1 alpha 35 A 90.5 - 22000 1:2 1:2 1:2 16.2 CCL4/MIP-1 beta 37 A 123 - 30000 1:2 1:2 1:2 5.8 CCL7/MCP-3/MARC 19 I 61.7 - 15000 1:2 1:2 1:2 3.2 CCL11/Eotaxin 51 J 61.7 - 15000 1:2 1:2 1:2 14.6 CCL22/MDC 36 I 53.5 - 13000 1:2 1:2 1:2 8.5 CD40 Ligand/TNFSF5 74 J 206 - 50000 1:2 1:2 1:2 34.7 CX3CL1/Fractalkine 46 L 617 - 150000 1:2 1:2 1:2 64.8 CXCL1/GRO alpha/KC/CINC-1 44 C 49.8 - 12000 1:2 1:2 1:2 5.3 CXCL2/GRO beta/MIP-2/CINC-3 27 K 45.3 - 11,000 1:2 1:2 1:2 7.86 CXCL10/IP-10/CRG-2 21 C 1.65 - 400 1:2 1:2 1:2 1.18 EGF 25 C 8.23 - 2000 1:2 1:2 1:2 8.9 FGF basic/FGF2/bFGF 47 B 1.77 - 450 1:2 1:2 1:2 6.5 Flt-3 Ligand/FLT3L 77 H 15.2 - 3700 1:2 1:2 1:2 1.50 G-CSF 54 B 24.7 - 6000 1:2 1:2 1:2 4.1 GM-CSF 66 B 12.3 - 3000 1:2 1:2 1:2 4.1 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-1 alpha/IL-1F1 38 B 4.94 - 1200 1:2 1:2 1:2 0.9 IL-1 beta/IL-1F2 28 A 17.7 - 4300 1:2 1:2 1:2 0.8 IL-1ra/IL-1F3 30 A 28.8 - 7000 1:2 1:2 1:2 18.0 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-3 15 H 78.2 - 19000 1:2 1:2 1:2 11.6 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-7 20 K 5.14 - 1250 1:2 1:2 1:2 0.41 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-12 p70 34 B 144 - 35000 1:2 1:2 1:2 20.2 IL-15 52 J 6.3 - 1550 1:2 1:2 1:2 1.01 IL-17/IL-17A 42 B 13.2 - 3200 1:2 1:2 1:2 1.8 Lymphotoxin-alpha/TNF-beta 45 N 7 - 1700 1:2 1:2 1:2 1.52 PDGF-AA 56 J 4.94 - 1200 1:50 1:2 1:2 0.747 PDGF-BB 14 H 12.3 - 3000 1:50 1:50 1:2 0.2 TGF-alpha 63 L 6.17 - 1500 1:2 1:2 1:2 0.820 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 VEGF-A 26 A 11.5 - 2800 1:2 1:2 1:2 0.99 Human Cytokine/Chemokine 2 Panel 20-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) , 15 R 4.57 - 1110 1:2 1:2 1:2 0.119 CCL8/MCP-2 37 D 15.2 - 3700 1:2 1:2 1:2 1.8 CCL13/MCP-4 38 D 4.94 - 1200 1:2 1:2 1:2 0.40 CCL17/TARC 63 G 103 - 25000 1:2 1:2 1:2 8.9 CCL24/Eotaxin-2/MPIF-2 34 K 20.6 - 5000 1:2 1:2 1:2 1.34 CCL26/Eotaxin-3 45 I 12.3 - 3000 1:2 1:2 1:2 1.6 CCL27/CTACK 74 H 7.41 - 1800 1:2 1:2 1:2 0.421 CXCL5/ENA-78 52 B 52.3 - 12500 1:2 1:2 1:2 8.2 CXCL13/BLC/BCA-1 28 D 18.5 - 4500 1:2 1:2 1:2 11.5 IL-16 25 R 40.6 - 9900 1:2 1:2 1:2 1.35 IL-21 65 R 29.4 - 7100 1:2 1:2 1:2 0.869 IL-23 76 C 144 - 35000 1:2 1:2 1:2 11.4 IL-28A/IFN-lambda 2 12 C 144 - 35000 1:2 1:2 1:2 8.6 IL-33 14 C 12.3 - 3000 1:2 1:2 1:2 1.8 LIF 33 K 24.7 - 6000 1:2 1:2 1:2 9.31 SCF/c-kit Ligand 27 F 16.5 - 4000 1:2 1:2 1:2 1.4 Thrombopoietin/Tpo 55 B 370 - 90000 1:2 1:2 1:2 24.8 TRAIL/TNFSF10 72 K 28.8 - 7000 1:2 1:2 1:2 9.65 TSLP 48 4 2.88 - 700 1:2 1:2 1:2 0.432 CCL21/6Ckine 65 M 41.2 - 10000 1:2 1:2 1:2 9.06 Human T Cell Panel 21-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CCL3/MIP-1 alpha 35 A 90.5 - 22000 1:2 1:2 1:2 16.2 CCL4/MIP-1 beta 37 A 123 - 30000 1:2 1:2 1:2 5.8 CCL20/MIP-3 alpha 33 D 8.23 - 2000 1:2 1:2 1:2 3.4 CX3CL1/Fractalkine 46 L 617 - 150000 1:2 1:2 1:2 64.8 CXCL11/I-TAC 63 B 24.7 - 6000 1:2 1:2 1:2 7.8 GM-CSF 66 B 12.3 - 3000 1:2 1:2 1:2 4.1 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-1 beta/IL-1F2 28 A 17.7 - 4300 1:2 1:2 1:2 0.8 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-7 20 K 5.14 - 1250 1:2 1:2 1:2 0.41 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-12 p70 56 B 144 - 35000 1:2 1:2 1:2 20.2 IL-13 47 G 453 - 110000 1:2 1:2 1:2 36.6 IL-17/IL-17A 42 B 13.2 - 3200 1:2 1:2 1:2 1.8 IL-21 65 R 29.4 - 7100 1:2 1:2 1:2 0.869 IL-23 76 C 144 - 35000 1:2 1:2 1:2 11.4 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 Human Th1/Th2 Panel 9-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) GM-CSF 46 B 12.3 - 3000 1:2 1:2 1:2 4.1 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-12 p70 56 B 144 - 35000 1:2 1:2 1:2 20.2 IL-13 47 G 453 - 110000 1:2 1:2 1:2 36.6 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 Human Th17 Panel 21-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CCL20/MIP-3 alpha 33 D 8.23 - 2000 1:2 1:2 1:2 3.4 GM-CSF 46 B 12.3 - 3000 1:2 1:2 1:2 4.1 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-1 beta/IL-1F2 28 A 17.7 - 4300 1:2 1:2 1:2 0.8 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-12 p70 56 B 144 - 35000 1:2 1:2 1:2 20.2 IL-13 47 G 453 - 110000 1:2 1:2 1:2 36.6 IL-15 63 J 6.3 - 1550 1:2 1:2 1:2 1.01 IL-17/IL-17A 42 B 13.2 - 3200 1:2 1:2 1:2 1.8 IL-17E/IL-25 30 K 547 - 133000 1:2 1:2 1:2 27.7 IL-21 65 R 29.4 - 7100 1:2 1:2 1:2 0.869 IL-27 25 M 309 - 75000 1:2 1:2 1:2 2.7 IL-28A/IFN-lambda 2 12 C 144 - 35000 1:2 1:2 1:2 8.6 IL-31 36 D 74.1 - 18000 1:2 1:2 1:2 9.7 IL-33 14 C 12.3 - 3000 1:2 1:2 1:2 1.8 Lymphotoxin-alpha/TNF-beta 45 N 7 - 1700 1:2 1:2 1:2 1.52 TNF-alpha 64 A 8.23 - 2000 1:2 1:2 1:2 1.2 Immuno-Oncology Checkpoint Panel 32-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CCL2/JE/MCP-1 25 A 30.9 - 7500 1:2 1:2 1:2 9.9 CCL3/MIP-1 alpha 35 A 90.5 - 22000 1:2 1:2 1:2 16.2 CCL4/MIP-1 beta 37 A 123 - 30000 1:2 1:2 1:2 5.8 CD27/TNFRSF7 67 E 103 - 25000 1:2 1:2 1:2 4.8 CD40/TNFRSF5 76 E 20.6 - 5000 1:2 1:2 1:2 2.1 CD40 Ligand/TNFSF5 74 J 206 - 50000 1:2 1:2 1:2 34.7 CXCL2/GRO beta/MIP-2/CINC-3 27 K 45.3 - 11,000 1:2 1:2 1:2 7.86 CXCL5/ENA-78 52 B 52.3 - 12500 1:2 1:2 1:2 8.2 CXCL6/GCP-2 21 K 32.9 - 8000 1:2 1:2 1:2 3.02 Galectin-1 51 L 1750 - 425000 1:2 1:2 1:2 632 Galectin-3 34 D 16.9 - 4100 1:50 1:50 1:2 1.7 Galectin-9 18 M 82.3 - 20000 1:2 1:2 1:2 29.0 GITR/TNFRSF18 61 O 83.4 - 20000 1:2 1:2 1:2 11.1 IFN-gamma 29 A 2.67 - 650 1:2 1:2 1:2 0.22 IL-1 alpha/IL-1F1 38 B 4.94 - 1200 1:2 1:2 1:2 0.9 IL-1 beta/IL-1F2 28 A 17.7 - 4300 1:2 1:2 1:2 0.8 IL-2 43 B 30.9 - 7500 1:2 1:2 1:2 1.8 IL-3 15 H 78.2 - 19000 1:2 1:2 1:2 11.6 IL-4 39 B 17.3 - 4200 1:2 1:2 1:2 9.3 IL-5 53 B 6.17 - 1500 1:2 1:2 1:2 0.5 IL-6 13 A 3.25 - 790 1:2 1:2 1:2 1.7 IL-7 20 K 5.14 - 1250 1:2 1:2 1:2 0.41 IL-8/CXCL8 48 A 4.12 - 1000 1:2 1:2 1:2 1.8 IL-10 22 A 4.1 - 1000 1:2 1:2 1:2 1.6 IL-12 p70 56 B 144 - 35000 1:2 1:2 1:2 20.2 IL-15 63 J 6.3 - 1550 1:2 1:2 1:2 1.01 IL-17/IL-17A 42 B 13.2 - 3200 1:2 1:2 1:2 1.8 PD-L1/B7-H1 14 K 4.53 - 1100 1:2 1:2 1:2 4.53 TNF-alpha 12 A 8.23 - 2000 1:2 1:2 1:2 1.2 CCL7/MCP-3/MARC 37 I 61.7 - 15000 1:2 1:2 1:2 3.2 IL-13 47 G 453 - 110000 1:2 1:2 1:2 36.6 MICA 12 F 65.8 - 16000 1:2 1:2 1:2 5.4 Human Kidney Injury Panel 3-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) beta 2-Microglobulin 13 K 74.1 - 18000 1:4,000 1:4,000 1:2 17.2 RBP4/Retinol-Binding Protein 4 61 J 1,012 - 246,000 1:4,000 1:4,000 1:2 231 Uromodulin 64 Q 778 - 190000 1:50 1:50 1:2 36.6 Human MMP Panel 1 3-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) MMP-3 15 A 82.3 - 20000 1:2 1:2 1:2 5.3 MMP-12 34 O 41.2 - 10000 1:2 1:2 1:2 7.1 MMP-13 33 O 144 - 35000 1:2 1:2 1:2 19.0 * Use heparin plasma as EDTA and citrate plasma samples are not recommended as anticoagulants due to their chelating properties. Human MMP Panel 2 4-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) MMP-1 19 A 49.4 - 12000 1:2 1:2 1:2 2.7 MMP-7 21 A 327 - 79,520 1:2 1:2 1:2 23.2 MMP-9 14 A 123 - 30000 1:50 1:50 1:2 13.6 MMP-10 66 R 53.7 - 13000 1:2 1:2 1:2 1.30 * Use heparin plasma as EDTA and citrate plasma samples are not recommended as anticoagulants due to their chelating properties. Human Sepsis Panel 1 7-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) C-Reactive Protein/CRP 62 B 119 - 29000 1:200 1:200 1:2 116 Fas/TNFRSF6/CD95 73 E 144 - 35000 1:2 1:2 1:2 3.2 ICAM-1/CD54 61 B 3200 - 775,000 1:2 1:2 1:2 87.9 MIF 21 F 556 - 135000 1:2 1:2 1:2 38.8 Serpin E1/PAI-1 43 D 18.1 - 4400 1:200 1:200 1:2 0.7 VCAM-1/CD106 57 B 8850 - 2150000 1:2 1:2 1:2 238 Fas Ligand/TNFSF6 39 D 8.23 - 2000 1:2 1:2 1:2 1.2 *Fas/TNFRSF6/CD95 cannot be multiplexed with Fas Ligand/TNFSF6 Human Sepsis Panel 2 7-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CCL3/MIP-1 alpha 35 A 90.5 - 22000 1:2 1:2 1:2 16.2 CCL4/MIP-1 beta 37 A 123 - 30000 1:2 1:2 1:2 5.8 Granzyme B 57 L 19.3 - 4700 1:2 1:2 1:2 1.42 IL-1 alpha/IL-1F1 38 B 4.94 - 1200 1:2 1:2 1:2 0.9 IL-8/CXCL8 18 A 4.12 - 1000 1:2 1:2 1:2 1.8 MMP-8 27 A 226 - 55000 1:2 1:2 1:2 34.2 Procalcitonin 39 L 8.23 - 2000 1:2 1:2 1:2 0.967 Human Soluble Cytokine Receptor Panel 11-plex Analyte BeadRegion StandardCocktail Standard Curve(pg/mL) Serum Plasma CellCulture Sensitivity(pg/mL) CD30/TNFRSF8 61 E 2.06 - 500 1:2 1:2 1:2 0.100 CD25/IL-2R alpha 47 I 26.7 - 6500 1:2 1:2 1:2 1.3 gp130 13 H 412 - 100000 1:2 1:2 1:2 11.2 IL-1 RII 62 E 206 - 50000 1:2 1:2 1:2 18.0 IL-4R alpha 22 R 2.42 - 600 1:2 1:2 1:2 0.290 IL-6R alpha 27 I 50 - 64,100 1:2 1:2 1:2 0.6 RAGE/AGER 45 D 123 - 30000 1:2 1:2 1:2 7.2 TNF RI/TNFRSF1A 66 E 49.4 - 12000 1:2 1:2 1:2 41.0 VEGFR1/Flt-1 38 Y 35.8 - 8700 1:2 1:2 1:2 5.68 VEGFR2/KDR/Flk-1 12 O 144 - 35000 1:2 1:2 1:2 7.40 VEGFR3/Flt-4 57 E 70 - 17000 1:2 1:2 1:2 2.7 Human Cell Pyroptosis Panel, 6-plex Item Name Detection range Sensitivity Alternative Names Caspase 1 0.02-20ng/mL <0.007 ng/mL ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase Caspase 4 0.01-10ng/mL <0.003 ng/mL ICE(rel)II; ICH2; TX; Protease ICH-2; Cysteinyl Aspartate Specific Proteinases 4; Apoptosis-Related Cysteine Peptidase Caspase 5 0.01-10ng/mL <0.003 ng/mL ICE(rel)III; Cysteinyl Aspartate Specific Proteinases 5; Apoptosis-Related Cysteine Peptidase; Protease ICH-3; Protease TY NLR Family, Pyrin Domain Containing Protein 3 0.02-20ng/mL <0.007 ng/mL AVP; AGTAVPRL; AII; AII/AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCU; MWS; NALP3; PYPAF1; Cryopyrin; Cold Induced Autoinflammatory Syndrome 1; Caterpiller-Like Receptor 1.1 Gasdermin D 0.02-20ng/mL <0.007 ng/mL GSDMDC1; DF5L; Gasdermin Domain Containing 1 NLR Family, CARD Domain Containing Protein 4 0.01-10ng/mL <0.003 ng/mL CARD12; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; IPAF; Ice protease-activating factor; Caspase Recruitment Domain Family Member 12; NOD-Like Receptor C4 Human Mucosal Immunity Panel, 3-plex Item Name Detection range Sensitivity Alternative Names Mucin 5 Subtype AC 4.88-5000pg/mL <1.627 pg/mL TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein Mucin 5 Subtype B 0.02-20ng/mL <0.007 ng/mL MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin Secretory Immunoglobulin A 19.53-20000pg/mL <6.51 pg/mL S-IgA Human Osteogenic Factor Panel, 8-plex Item Name Detection range Sensitivity Alternative Names Sclerostin 0.02-20ng/mL <0.007 ng/mL VBCH; Sclerosteosis Osteoprotegerin 0.01-10ng/mL <0.003 ng/mL TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor Bone Morphogenetic Protein 2 0.98-1000pg/mL <0.327 pg/mL BMP2A; BMP-2A; Hemochromatosis Modifier Osteocalcin 0.04-40ng/mL <0.013 ng/mL BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein Runt Related Transcription Factor 2 0.01-10ng/mL <0.003 ng/mL AML3; CBFA1; CCD1; OSF2; PEBP2A; Acute myeloid leukemia 3 ; Core-binding factor alpha-1; Osteoblast-specific transcription factor 2; Polyomavirus enhancer-binding protein 2 A Klotho 0.98-1000pg/mL <0.327 pg/mL KLa; Klotho Alpha Matrix Gla Protein 0.04-40ng/mL <0.013 ng/mL MGLAP; GIG36; Cell growth-inhibiting gene 36 protein S100 Calcium Binding Protein A11 0.98-1000pg/mL <0.327 pg/mL S100-A11; S100C; MLN70; Protein S100-C; Calgizzarin; Metastatic lymph node gene 70 protein; Protein S100-A11, N-terminally processed Human Claudin Panel, 8-plex Item Name Detection range Sensitivity Alternative Names Claudin 1 0.01-10ng/mL <0.003 ng/mL CLD1; ILVASC; SEMP1; Senescence-Associated Epithelial Membrane Protein 1 Claudin 2 0.01-10ng/mL <0.003 ng/mL SP82 Claudin 3 0.02-20ng/mL <0.007 ng/mL C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2 Claudin 4 0.01-10ng/mL <0.003 ng/mL CPE-R; CPER; CPETR; CPETR1; WBSCR8; hCPE-R; Clostridium Perfringens Enterotoxin Receptor 1; Williams-Beuren Syndrome Chromosomal Region 8 Protein Claudin 5 1.95-2000pg/mL <0.65 pg/mL AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS Claudin 6 0.01-10ng/mL <0.003 ng/mL Skullin Claudin 8 0.01-10ng/mL <0.003 ng/mL Claudin 18 0.01-10ng/mL <0.003 ng/mL SFTP-J; SFTPJ; SP-J; SPJ; Surfactant Protein J; Surfactant Associated Protein J Human Amyloid Beta and Tau Panel 4-plex Sensitivity • Aβ1-40: 10.2 pg/mL (MinDC+2SD)
• Aβ1-42: 2.5 pg/mL (MinDC+2SD)
• tTau: 14.2 pg/mL (MinDC+2SD)
• pTau181: 1.5 pg/mL (MinDC+2SD)Standard Curve Range • Aβ1-40: 21-15000 pg/mL
• Aβ1-42: 2-2000 pg/mL
• tTau: 11-8000 pg/mL
• pTau181: 0.7-500 pg/mLApplication Notes • A 1:2 sample dilution is recommended for extracted CSF samples. Human Neuroscience Panel1 6-plex Sensitivity • See protocol for sensitivities of individual analytes Standard Curve Range • GFAP 12-50,000pg/mL
• UCHL1 20-80,000pg/mL
• DJ1 61-250,000pg/mL
• NSE 15-60,000 pg/mL
• alpha-synuclein 244-1,000,000 pg/mL
• TG2 61-250,000 pg/mLHuman Neuroscience Panel2 6-plex Sensitivity • Angiogenin: 5.8 pg/mL
• ApoE4: 162.8 pg/mL
• FABP3: 14.3 pg/mL
• Ferritin: 6.1 pg/mL
• Neurogranin: 19.9 pg/mL
• TREM2: 11.0 pg/mLStandard Curve Range • Angiogenin: 2 - 10,000 pg/mL
• ApoE4: 244 - 1,000,000 pg/mL
• FABP3: 24 - 100,000 pg/mL
• Ferritin: 6 - 25,000 pg/mL
• Neurogranin: 5 - 20,000 pg/mL
• TREM2: 15 - 50,000 pg/mLApplication Notes • For Plasma/Serum a 1:10 dilution is recommended for the optimal detection of the analytes FABP3, Ferritin, Neurogranin, and TREM2.
• For Plasma/Serum a 1:200 dilution for ApoE4 and Angiogenin is recommended.
• Because the sample dilutions differ for Plasma/Serum samples ApoE4 and Angiogenin cannot be plexed in the same assay as FABP3, Ferritin, Neurogranin, and TREM2.
• CSF samples require a 1:10 dilution and so may all be plexed together
• Cell/Tissue culture supernatants or homogenates may be used neat or diluted in appropriate control medium.Human Neurodegenerative Disease Panel1 6-plex Sensitivity • See protocol for sensitivities of individual analytes Standard Curve Range • Apo CIII: 0.01 – 40 ng/mL
• Apo E, Complement C3: 0.05 – 200 ng/mL
• Complement Factor H: 0.3 – 1,000 ng/mL
• α-2-Macroglobulin: 0.5 – 2,000 ng/mL
• Apo A1: 0.7 – 3,000 ng/mLApplication Notes • This assay requires 25 μL of 1:40,000 diluted serum/plasma or 1:400 diluted CSF per well. Human Neurodegenerative Disease Panel2 6-plex Sensitivity • See kit protocol for individual sensitvities Standard Curve Range • α-1-Antitrypsin: 0.24-1,000 ng/mL
• Complement C4: 0.24-1,000 ng/mL
• CRP: 0.10-50 ng/mL
• MIP-4: 4.88-20,000 ng/mL
• PEDF: 0.05-200 ng/mL
• SAP: 0.12-500 ng/mLApplication Notes • This assay requires 25 μL of 1:2,000 diluted serum/plasma or 1:20 diluted CSF per well. Human Neurodegenerative Disease Panel3 10-plex Sensitivity • See kit protocol for individual sensitvities Standard Curve Range • BDNF 2.4-10,000 pg/mL
• Cathepsin D 24-100,000 pg/mL
• MPO 24-100,000 pg/mL
• NCAM 24-100,000 pg/mL
• PAI-1 (total) 2.4-10,000 pg/mL
• PDGF-AA 2.4-10,000 pg/mL
• PDGF-AB/BB 24-100,000 pg/mL
• RANTES 2-10,000 pg/mL
• sICAM-1 24-100,000 pg/mL
• sVCAM-1 61-250,000 pg/mLApplication Notes • This assay requires 25 μL of 1:100 diluted serum/plasma or neat CSF per well. Human Neurodegenerative Disease Panel4 5-plex Sensitivity • See kit protocol for individual sensitvities Standard Curve Range • Amyloid β40: 3 - 2,500 pg/mL
• Amyloid β42: 27 - 20,000 pg/mL
• GDNF: 7 - 5,000 pg/mL
• sRAGE: 21 - 15,000 pg/mL
• S100B: 14 - 10,000 pg/mLApplication Notes • This assay requires 25 μL 1:3 diluted CSF per well. Human Neurological Disorders Panel3 7-plex Sensitivity • See kit protocol for individual sensitvities Standard Curve Range • AGT: 1.37 – 1,000 ng/mL
• Contactin-1: 0.08 – 60 ng/mL
• Fetuin A: 4.12 – 3,000 ng/mL
• Kallikrein-6: 0.04 – 30 ng/mL
• Osteopontin (OPN): 0.14 – 100 ng/mL
• SOD1: 0.04 – 30 ng/mL
• SOD2: 0.03 – 25 ng/mLApplication Notes • CSF samples: 1:100 dilution in kit Assay Buffer for all analytes.
• Serum and Plasma samples: 1:50 dilution in kit Assay Buffer for all analytes except Fetuin A.
NOTE: Fetuin A cannot be assayed along with other analytes in serum/plasma samples. Fetuin A should be diluted 1:4000 in the kit Assay Buffer and analyzed as a singleplex for serum and plasma samples.Human Neuropeptide Panel 5-plex Sensitivity • 11 - 479 pg/mL (MinDC+2SD).
• Refer to kit protocol for sensitivities of individual biomarkers.Standard Curve Range • α-MSH: 27 – 20,000 pg/mL
• β-Endorphine: 69 – 50,000 pg/mL
• Neurotensin: 41 – 30,000 pg/mL
• Oxytocin: 55 – 40,000 pg/mL
• Substance P: 7 – 5,000 pg/mLApplication Notes • This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well. Human Circadian/Stress Panel 2-plex Sensitivity • Cortisol: 686 pg/mL (MinDC+2SD)
• Melatonin: 4.3 pg/mL (MinDC+2SD)Standard Curve Range • Cortisol: 686 – 500,000 pg/mL
• Melatonin: 3.4 – 2,500 pg/mLApplication Notes • This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture supernatant per well.
• Sample extraction protocols are required for serum/plasma samples. Other sample types (e.g. CSF, culture supernatant, saliva, etc.) do not require sample extraction.
• A 1:4 sample dilution is recommended for extracted serum/plasma samples.Human Exosome Characterization Panel 9-plex Sensitivity • Refer to kit protocol for sensitivities for individual analytes. Standard Curve Range • Calreticulin, GAPDH: 24-100,000 pg/mL
• CD9: 0.4-1,500 ng/mLmL
• CD63: 122-500,000 pg/mL
• CD81: 0.5-2,000 ng/mL
• Syntenin-1: 0.9-3,500 ng/mL
• Flotillin-1: 0.6-2,500 ng/mL
• TSG101: 49-200,000 pg/mL
• Argonaute-2: qualitative assay, no standard rangeApplication Notes • This is an overnight assay.
• Each assay requires 25 µL per well of isolated exosomes.
• Argonaute-2 (Ago2) is a qualitative assay with the MCF7:IGF-1 Cell Lysate included as a control. All other analytes are for quantitative analysis.
• Argonaute-2 (Ago) is a static bead component and will always be included in the kit format. The remaining analytes are configurable.
• To test purity of EV preparation, the MILLIPLEX® Human Apolipoprotein 6-Plex Panel (Cat. No. APOMAG-62K) can be used.
• To asses the functional component of EVs, the MILLIPLEX® Human Cytokine/Chemokine Growth Factor Panel A (Cat. No. HCYTA-60K), Panel B (HCYTB-60K), and Multi-Species TGFβ Panel (Cat. No. TGFBMAG-64K-03) can be used.
• Following sample collection and preparation, exosomes can now be isolated and lysed according to your lab’s established protocol. We recommend Cell Signaling Lysis Buffer (Cat. No. 43-040).
• Each lab may use its preferred lysis buffer but must predetermine that the assay will not be negatively affected by the lysis buffer.
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